We use cells grown and manipulated in culture (gene inductions, RNA interference, CRISPR/Cas9) as our model systems. We use fluorescence microscopy (immunofluorescence, fluorescence in situ hybridization) to study chromatin localization. We apply classical molecular biology techniques such as cloning, real-time quantitative PCR, northern and western blotting, RNA and protein immunoprecipitation to identify and study underlying molecular mechanisms that regulate spatial chromatin organization and gene expression. We use both classical genomics (Chromatin immunoprecipitation, RNA-seq) and cutting-edge 3D genomics approaches (Hi-C, 5C) to map chromatin composition (epigenomics), activity, and folding. In collaboration with other groups, we integrate our findings computationally to uncover when, how, and why spatial genome organization affects the expression of genes.